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dmem cell culture medium  (Beijing Solarbio Science)


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    Beijing Solarbio Science dmem cell culture medium
    Dmem Cell Culture Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+cell+culture+medium/Medium/10__1016_slash_j__jddst__2026__108137-83-0-14
    Average 99 stars, based on 10877 article reviews
    dmem cell culture medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: A dual-targeting and pH/NIR-stimuli-responsive nanoassembly for enhanced cuproptosis, chemo-, and phototherapy in colorectal cancer
    Article Snippet: Cuproptosis has gained significant attention in cancer therapy.. Use of copper-overloaded combination therapy that employs a variety of cell death mechanisms has emerged as a promising avenue in the treatment of cancer.. However, integrating different modes in a system with optimal synergy and minimal side effects remains a challenge.

    Article Title: Blocking the ERK1/2 signal pathway can inhibit S100A12 induced human aortic smooth muscle cells damage.
    Article Snippet: Increased levels of S100A12 and activated matrix metalloproteinase 2/9 (MMP-2/9) produced by human aortic smooth muscle cells (HASMCs) have recently implicated in the development of thoracic aortic disease.. In the present study, we investigated the effect of S100A12 on HASMCs and identified the intracellular signal pathways involved by western blot.. The results were shown that up-expression of S100A12 in HASMCs induced cell apoptosis and inhibited cell proliferation.

    Article Title: Self-Assembled PEGylated Nanocubes Based on Hydrophobic Camptothecin and Doxorubicin for Combinational Therapy of Colorectal Cancer.
    Article Snippet: Nanoassemblies based on drug conjugates with high drug loading efficiency and stability have been regarded as promising candidates for the next generation of drug formulations.. However, they are mostly amphiphilic.. Here, a dual-hydrophobic drug conjugate-based nanoassembly has been created for enhanced synergistic antiproliferation against colorectal cancer cells.

    Article Title: Kaji-Ichigoside F1 and Rosamultin Protect Vascular Endothelial Cells against Hypoxia-Induced Apoptosis via the PI3K/AKT or ERK1/2 Signaling Pathway
    Article Snippet: LY294002 was obtained from MedChemExpress (New Jersey, USA); PD98059 was obtained from Selleckchem (Houston, USA). .. HyClone foetal bovine serum was obtained from Thermo Scientific (Massachusetts, USA), and DMEM cell culture medium was obtained from Solarbio (Beijing, China). .. Primary antibodies against p-ERK1/2, ERK1/2, p-AKT, AKT, p-NF- κ B, NF- κ B, Bcl-2, Bax, Cyt C, cleaved caspase-9, and cleaved caspase-3 were bought from Cell Signaling Technology (Boston, USA).

    Article Title: The host protein CCT8 interacts with PB2 protein of H9N2 AIV and facilitates virus replication.
    Article Snippet: In recent years, the poultry industry and public health safety have been seriously threatened by low pathogenic avian influenza virus (AIV), such as H9N2.. PB2, as a member of RNA polymerase complex of avian influenza virus, plays a crucial role in viral replication and the infection process.. To elucidate the mechanism of H9N2 influenza virus infection in host cells, PB2 protein was used as target protein to screen the interacting host proteins through CO-IP, silver staining, and protein mass spectrometry.

    Article Title: A Redox Cleavable and Targeting Nanoassembly Based on CPT/DOXY Conjugates for Synergistically Enhanced Chemo/Photodynamic/Photothermal Combined Tumor Therapy.
    Article Snippet: Combined use of different drugs and therapy modes has been effective in improving tumor therapy efficiency and reducing side effects.. However, synergistic anticancer efficiency and highly responsive drug release still remain challenging.. Herein, we found that camptothecin (CPT), doxycycline (DOXY), and IR780 exhibit a synergistic anticancer effect on CT26 colorectal cancer cells.

    cDNA Synthesis:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    SYBR Green Assay:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Saline:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    TUNEL Assay:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Membrane:

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells.
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), Lascorbic acid, Silver Nitrate (AgNO3), Gold (III) chloride solution (HAuCl4.3H2O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced AR TIC LE IN PR ES S reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Article Title: Synergistic chemo-photothermal therapy using doxorubicin-loaded gold nanorods for enhanced apoptosis in lung cancer cells
    Article Snippet: Cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH 4 ), L-ascorbic acid, Silver Nitrate (AgNO 3 ), Gold (III) chloride solution (HAuCl 4 .3H 2 O), β-cyclodextrin (β-CD), p-toluenesulfonyl chloride (TsCl), and thiourea were purchased from Sigma-Aldrich. .. Deionized water, Chloroform, Ethanol, Trizol reagent, DEPC-treated water, DMEM cell culture medium, Fetal bovine serum (FBS), Penicillin-streptomycin, Doxorubicin (Cell culture Solarbio), cDNA synthesis kit (Yekta Tajhiz), SYBR green, DMSO (for cell culture), Trypsin-EDTA (0.25%), Phosphate-buffered saline (PBS), Tris buffer, TUNEL Assay kit (Roche), DAPI (9542D-Sigma), Sodium deoxycholate, NaCl, EDTA, HCl, Phosphoric acid, Coomassie Blue G250, NP-40, Triton, APS (Ammonium Persulfate), Bromophenol blue, β-mercaptoethanol, Distilled water, Glycerol, PVDF membrane, Bis-acrylamide, Acrylamide, Tetramethylethylenediamine (TEMED), Caspase-8 (1C12) antibody, Caspase-3 (D3R6Y) Rabbit mAb, Actin (2A3): sc-517,582, Donkey anti-goat IgG-HRP, Caspase-9 antibody (human-specific), β-Actin (2A3): sc-517,582،Caspase-3 (D3R6Y) Rabbit mAb ،Caspase-8 (1C12) Mouse mAb, ECL advanced reagents, Tween 20 (0.1% v/v). .. Human lung cancer cell line A549 was obtained from the Pasteur Institute of Iran.

    Lysis:

    Article Title: The host protein CCT8 interacts with PB2 protein of H9N2 AIV and facilitates virus replication.
    Article Snippet: In recent years, the poultry industry and public health safety have been seriously threatened by low pathogenic avian influenza virus (AIV), such as H9N2.. PB2, as a member of RNA polymerase complex of avian influenza virus, plays a crucial role in viral replication and the infection process.. To elucidate the mechanism of H9N2 influenza virus infection in host cells, PB2 protein was used as target protein to screen the interacting host proteins through CO-IP, silver staining, and protein mass spectrometry.

    Centrifugation:

    Article Title: The host protein CCT8 interacts with PB2 protein of H9N2 AIV and facilitates virus replication.
    Article Snippet: In recent years, the poultry industry and public health safety have been seriously threatened by low pathogenic avian influenza virus (AIV), such as H9N2.. PB2, as a member of RNA polymerase complex of avian influenza virus, plays a crucial role in viral replication and the infection process.. To elucidate the mechanism of H9N2 influenza virus infection in host cells, PB2 protein was used as target protein to screen the interacting host proteins through CO-IP, silver staining, and protein mass spectrometry.



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